Special Techniques Applicable to Bone Marrow Diagnosis
ثبت نشده
چکیده
A Perls’ or Prussian blue stain (Figs 2.1 and 2.2) demonstrates haemosiderin in bone marrow macrophages and within erythroblasts. Consequently, it allows assessment of both the amount of iron in reticulo-endothelial stores and the availability of iron to developing erythroblasts. Assessment of storage iron requires that an adequate number of fragments are obtained. A bone marrow film or squash will contain both intracellular and extracellular iron, the latter being derived from crushed macrophages. It is usual to base assessment of iron stores mainly on intracellular iron since iron stains are prone to artefactual deposits and it can be difficult to distinguish between extracellular iron and artefact. Iron stores may be assessed as normal, decreased or increased, or may be graded as 1+ to 6+ as shown in Table 2.1, grades of 1+ to 3+ being considered normal. Alternatively, iron stores may be graded as 1+ to 4+ [3,4]. Examination of a Perls’ stain of a bone marrow film allows adequate assessment of erythroblast iron as long as a thinly spread area of the film is examined with optimal illumination. A proportion of normal erythroblasts have a few (one to five) fine iron-containing granules randomly distributed in TWO
منابع مشابه
The Role of Bone Marrow Aspiration and Bone Marrow Biopsy in Diagnosis of Bone Marrow Metastases
Background: Bone marrow is the site of many malignant disorders and it is one of the common places for solid tumors to metastasize. Examination of the bone marrow aspirate and biopsy is a routine procedure performed for assessment of various conditions such as cytopenias, hematologic neoplasms, nonmalignant disorders and metastatic neoplasms. Methods: The patients were referred to the Hema...
متن کاملBeware of Bone Marrow: Incidental Detection and Primary Diagnosis of Solid Tumours in Bone Marrow Aspiration and Biopsies; A Study of 22 Cases
Background & objective: Introduction: First detection of any solid tumour as metastatic deposits in bone marrow directs clinicians to start searching for the primary tumour. Detection of bone marrow metastasis determines the stage of the malignancy, prognosis, mode of treatment, chemotherapeutic response and follow-up in case of relapse. The aim of the current...
متن کاملEvaluation of DAT in the Diagnosis of Visceral Leishmaniasis in Children Hospitals in Tabriz from 1992 to 1996
Since the bone marrow puncture and its study is a time-consuming and costly process and in addition, the direct agglutination test has a high sensitivity and is easier and quicker to perform for the diagnosis of Kala-azar, and considering the less value of bone marrow puncture for the definite diagnosis of endemic hepatosplenomegaly in Meshkinshahr, this study was performed to determine the dia...
متن کاملA CAD System Framework for the Automatic Diagnosis and Annotation of Histological and Bone Marrow Images
Due to ever increasing of medical images data in the world’s medical centers and recent developments in hardware and technology of medical imaging, necessity of medical data software analysis is needed. Equipping medical science with intelligent tools in diagnosis and treatment of illnesses has resulted in reduction of physicians’ errors and physical and financial damages. In this article we pr...
متن کاملBone Marrow and Karyotype Findings of Patients with Pancytopenia in Southern Iran
Background: Pancytopenia is a manifestation of a wide range of disorders. The main prognostic factor for predicting outcome and response to treatment is based on the underlying cause. To detect the root cause of this problem, depending on other accompanied signs or symptoms, the need for bone marrow examination and other advanced work ups is different at least at the practical level. This study...
متن کامل